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11365: Bordetella pertussis/parapertussis DNA, Qualitative, Real-Time PCR Print View
Source      
B. pertussis DNA RT-PCR     
B. parapertussis DNA RT-PCR     

PRIMARY
1 NP Aspirate/Wash 1 (0.3) mL   Room Temperature - 48 Hour(s)/Refrigerated - 8 Day(s)/Frozen - 30 Day(s)  
ALTERNATE
  Nasal Swab - Amies Liquid   Frozen - 30 Day(s)/Refrigerated - 7 Day(s)/Room Temperature - 7 Day(s)  
  NP Aspirate/Wash in VCM   Frozen - 30 Day(s)/Refrigerated - 7 Day(s)/Room Temperature - 7 Day(s)  
  NP Aspirate/Wash in UTM   Refrigerated - 7 Day(s)/Frozen - 30 Day(s)/Room Temperature - 7 Day(s)  
  ESwab Amies   Room Temperature - 7 Day(s)/Refrigerated - 7 Day(s)/Frozen - 30 Day(s)  
  Nasal Swab in M4   Frozen - 30 Day(s)/Refrigerated - 7 Day(s)/Room Temperature - 7 Day(s)  

Bordetella pertussis is the cause of whooping cough that may occur in unimmunized individuals. B. parapertussis is a related organism that causes a similar, but milder, disease. Laboratory diagnosis may require both culture and serological confirmation, although culture is difficult.

Nasopharyngeal aspirates:
Instill 1-1.5 mL of nonbacteriostatic saline (pH 7.0) into one
nostril. Flush a plastic catheter or tubing with 2-3 mL of saline.
Insert the tubing into the nostril parallel to the palate. Aspirate
nasopharyngeal secretions. Repeat this procedure for the other
nostril. Combine aspirates into a sterile vial.

Nasopharyngeal swab:
Collection kits using Amies liquid, M4, VCM/UTM are acceptable. Swab
tips may be polyester, rayon, nylon flocked. Flexible shafts must be
used for proper collection. Accuracy of the test depends on the
collection of ciliated epithelial cells material. Half the distance
from the nostril to the base of the ear could be used as estimation of
the site of collection. Gently insert swab into the nostril. Keep the
swab near the septum floor of the nose while gently pushing into the
posterior nasopharynx. Rotate swab 2-3 times and hold in place for few
seconds for optimal collection. Collection of both nostrils is
recommended but not required.
Setup Schedule
Sunday-Saturday

Reported (Analytical Time)
Same day

CPT Code
87798x2

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Transport Temperature: Refrigerated Methodology: Real-Time Polymerase Chain Reaction (RT-PCR) Assay Category: LDT ASR CLASS 1 Reject Criteria: Collection tubes with Amies gel or charcoal containing media; samples collected using cotton or calcium alginate tipped swabs; rigid shaft swabs






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